New Query Example Query Toolbox FAQ Disclaimers Data Policy Imprint


the fast & easy way to design your primer

» Calculate annealing temperature of your primer.

» Calculate annealing temperature of your primer pair.

» Calculate the number of A or T nucleotides necessary to accomplish the designated annealing temperature.

» Change your sequence to complementary and reverse.



The Primerfox Toolbox requires JAVAScript. Please enable it in your browser, if calculation does not work.

© Stephan Fuchs, primerfox.com